Western blot

Recombinant GP was sourced from Nuffield Department of Medicine, Oxford University, Oxford, UK and was based on the Makona strain of EBOV. Nucleoprotein (NP) was purchased from Gentaur (MBS1206629, partial expression 488-739) and is based on Myinga EBOV. 

VP40 was based on Myinga strain of EBOV and was purchased from Gentaur (IT-014-011Ep). Proteins were heat denatured and loaded onto 4–12% BisTris gels and separated by size by SDS-PAGE. The proteins were then transferred to PVDF membrane and blocked overnight in block buffer (PBST buffer with 5% milk (w/v)).